3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Edición Especial Special Issue Noviembre 2020
179
MICROBIAL BIODEGRADATION OF POLYETHYLENE
OF LOW DENSITY, UNDER CONTROLLED THERMAL
CONDITIONS IN AIR LIFT BIO-REACTOR
Alexandra Milagros Hermoza Rojas
University Cesar Vallejo, (Perú).
E-mail: alexandrah@gmail.com ORCID: https://orcid.org/0000-0001-7468-8969
Jorge Jave Nakayo
Universidad Nacional Mayor de San Marcos - UNMSM, Lima, (Perú).
E-mail: jorge.jave@unmsm.edu.pe ORCID: https://orcid.org/0000-0003-3536-881X
Jorge Luis López Bulnes
Universidad Nacional Mayor de San Marcos - UNMSM, Lima, (Perú).
E-mail: jlopezb@unmsm.edu.pe ORCID: https://orcid.org/0000-0002-9583-1143
Vicenta Irene Tafur Anzualdo
National University Federico Villarreal, (Perú).
E-mail: itafur@unfv.edu.pe ORCID: https://orcid.org/0000-0002-1888-7848
Recepción:
18/09/2020
Aceptación:
02/11/2020
Publicación:
13/11/2020
Citación sugerida Suggested citation
Hermoza, A. M., Jave, J., López, J. L., y Tafur, V. I. (2020). Microbial biodegradation of polyethylene of
low density, under controlled thermal conditions in air lift bio-reactor. 3C Tecnología. Glosas de innovación
aplicadas a la pyme. Edición Especial, Noviembre 2020, 179-189. https://doi.org/10.17993/3ctecno.2020.
specialissue6.179-189
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Edición Especial Special Issue Noviembre 2020
180
https://doi.org/10.17993/3ctecno.2020.specialissue6.179-189
ABSTRACT
The present investigation seeks to identify new mechanisms that serve as tools for
the mitigation of plastic contamination through the biodegradation of low density
polyethylene using microorganisms of the species Pseudomona aeruginosa (bacteria) and
Aspergillus brazilensis (fungus) under controlled thermal conditions in an airlift bioreactor.
The methods used were 2 samples of low density polyethylene with concentrations of
50 mg/L and 2 samples of 100 mg/L deposited in an airlift bioreactor under controlled
thermal conditions with a duration of 7 days. As a result it was obtained that the species
Pseudomona aeruginosa (bacteria) reduced the low density polyethylene sample by 2% with
a concentration of 49 mg/L at a temperature of 21.8ºC with a pH of 6.5 and dissolved
oxygen (OD) of 6.8 mg/L, likewise the species Aspergillus brazilensis (fungus) reduced the
low density polyethylene sample by 7% reaching a concentration of 93 mg/L at 22.1ºC of
temperature, 7.14 of pH and 7.45 of dissolved oxygen (OD).
KEYWORDS
Air lift bioreactor, Biodegradation, Thermal conditions, Low density polyethylene (LDPE).
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Edición Especial Special Issue Noviembre 2020
181
https://doi.org/10.17993/3ctecno.2020.specialissue6.179-189
1. INTRODUCTION
As we know, plastics are a big problem nowadays, since we live in a world where people do
not have an adequate environmental conscience, unfortunately this generates a very negative
impact to our planet. Plastics have a low economic value in the market and are the most
used, therefore, the amount of plastic waste increases. In addition, he tells us that “In 2010,
8.07% of plastic waste was generated in our country and in 2011 9.85%, this indicates that
every year there is an increase in plastic waste. Plastic bags (low-density polyethylene) take
150 years to degrade, which is why we have been looking for alternatives to reduce the life
span of plastic, such as incineration, which causes health problems because it generates the
famous greenhouse gases (GHGs), in addition to the release of dioxins and furans (POPs)
that are highly carcinogenic according to the World Health Organization (WHO) (Barlow
et al., 2019).
Plastic waste is a worldwide problem since people use the bags only once, throw them away
and buy others again without realizing it; this is how pollution begins, causing damage
mainly to marine ecosystems, since fauna often confuses them with food. A study conducted
for MINAM in June 2012 indicates that Peruvians generate approximately 23,260 tons of
solid waste, which gives us a per capita production of 800 grams (Córdova et al., 2020).
A study released in 2015 tells us that sea turtles have been ingesting 52% of garbage, with
plastic waste such as bags being the most common (Inforegion, 2015).
Finally, according to the Peruvian newspaper El Comercio (2018), 79% of plastic waste
globally is found in dumps or thrown on the roads; only 9% is recycled and 12% is
incinerated.
2. MATERIALS AND METHODS
2.1. PLACE OF STUDY AND SAMPLING
In this stage the place to work was selected and was an informal dump located in the town
center of Santa Clara in the district of Ate in Lima in April, which presented a large
amount of plastic in an apparent state of degradation (Catto et al., 2016).